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培養基配方
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Pfizer Selective Enterococcus Agar (PSE Agar)
[所屬分類:培養基配方] [發布時間:2021-12-8] [發布人:網站管理員2] [閱讀次數:] [返回]
Pfizer Selective Enterococcus Agar
(PSE Agar)
山東拓普生物工程有限公司 培養基配方 //ljxinglong.com
Peptone C..........................................................17.0g
Agar ..................................................................15.0g
Bile....................................................................10.0g
NaCl....................................................................5.0g
Yeast extract........................................................5.0g
Peptone B............................................................3.0g
Esculin ................................................................1.0g
Sodium citrate.....................................................1.0g
Ferric ammonium citrate.....................................0.5g
NaN3 .................................................................0.25g
pH 7.1 ± 0.2 at 25°C
Caution: Sodium azide is toxic. Azides also react
with metals and disposal must be highly diluted.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dish
es or leave in tubes.
Use: For the selective isolation, cultivation, and enu
meration of Enterococcus species by the multiple
tube technique.



