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培養基配方
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Peptone Iron Agar
[所屬分類:培養基配方] [發布時間:2021-12-8] [發布人:網站管理員2] [閱讀次數:] [返回]
Peptone Iron Agar
山東拓普生物工程有限公司 培養基配方 //ljxinglong.com
Composition per liter:
Agar ..................................................................15.0g
Peptone .............................................................15.0g
Proteose peptone.................................................5.0g
Sodium glycerophosphate...................................1.0g
Ferric ammonium citrate.....................................0.5g
Na2S2O3 ............................................................0.08g
pH 6.7 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Add components to dis
tilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat and bring to boiling. Distribute
into tubes. Autoclave for 15 min at 15 psi pressure–
121°C. Allow tubes to cool in an upright position.
Use: For the cultivation and differentiation of microor
ganisms based on their ability to produce H2S. Microor
ganisms that produce H2S turn the medium black.



