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Oxidative-Fermentative Glucose Medium, Semisolid with NaCl (OF Glucose Medium, Semisolid with NaCl) (BAM M116)

[所屬分類:培養基配方] [發布時間:2021-12-8] [發布人:網站管理員2] [閱讀次數:] [返回]
Oxidative-Fermentative
Glucose Medium, Semisolid with NaCl
(OF Glucose Medium,
Semisolid with NaCl)
(BAM M116)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

NaCl..................................................................20.0g
Glucose .............................................................10.0g
Agar ....................................................................2.0g
Pancreatic digest of casein..................................2.0g
K2HPO4...............................................................0.3g
Bromthymol Blue dye.......................................0.08g
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dish
es or leave in tubes.
Use: For differentiating Gram-negative halophilic
Vibrio spp. based upon determining the oxidative and
fermentative metabolism of glucose. Bacteria that
ferment glucose turn the medium yellow. For
Campylobacter detection prepare half of the tubes
with glucose and half without.
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