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Oxford Medium (BAM M118)

[所屬分類:培養基配方] [發布時間:2021-12-8] [發布人:網站管理員2] [閱讀次數:] [返回]
Oxford Medium
(BAM M118)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Special peptone.................................................23.0g
LiCl...................................................................15.0g
Agar ..................................................................10.0g
NaCl....................................................................5.0g
Cornstarch...........................................................1.0g
Esculin ................................................................1.0g
Ferric ammonium citrate.....................................0.5g
Antibiotic inhibitor ....................................... 10.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from Oxoid.
Supplement Mix:
Composition per 10.0mL:
Cycloheximide....................................................0.4g
Colistin sulfate..................................................0.02g
Fosfomycin .......................................................0.01g
Acriflavine .......................................................5.0mg
Cefotetan..........................................................2.0mg
Ethanol (50% solution)................................. 10.0mL
Preparation of Supplement Mix: Add compo
nents to 10.0mL of ethanol. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, ex
cept supplement mix, to distilled/deionized water
and bring volume to 990.0mL. Mix thoroughly. Gen
tly heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 50°C. Aseptically
add 10.0mL of sterile supplement mix. Mix thor
oughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the isolation and cultivation of Listeria
monocytogenes from specimens containing a mixed
bacterial flora.
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