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Organic Acid Medium KP (Organic Acid Medium, Kauffmann and Petersen)

[所屬分類:培養基配方] [發布時間:2021-12-7] [發布人:網站管理員2] [閱讀次數:] [返回]
Organic Acid Medium KP
(Organic Acid Medium,
Kauffmann and Petersen)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Gelatin...............................................................10.0g
Bromthymol Blue ...........................................0.024g
Organic acid solution .................................. 100.0mL
pH 7.4 ± 0.2 at 25°C
Organic Acid Solution:
Composition per 100.0mL:
Organic acid......................................................10.0g
Preparation of Medium: Add organic acid to
distilled/deionized water and bring volume to
100.0mL. Sodium potassium D-tartrate, sodium cit
rate, or mucic acid may be used. Mix thoroughly
Preparation of Medium: Add components, ex
cept organic acid solution, to distilled/deionized water
and bring volume to 900.0mL. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 45°–50°C. Aseptically
add sterile organic acid solution. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and differentiation of mem
bers of the Enterobacteriaceae based on their ability
to utilize different organic acids as carbon source.
Bacteria that utilize tartrate, citrate, or mucate turn
the medium yellow.
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