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Ogawa TB Medium
[所屬分類:培養基配方] [發布時間:2021-12-7] [發布人:網站管理員2] [閱讀次數:] [返回]
Ogawa TB Medium
山東拓普生物工程有限公司 培養基配方 //ljxinglong.com
Composition per 300.0mL:
KH2PO4...............................................................1.0g
Homogenized whole egg ............................ 200.0mL
Glycerol .......................................................... 6.0mL
Malachite Green (2% solution)....................... 6.0mL
pH 6.5 ± 0.2 at 25°C
Homogenized Whole Egg:
Composition per liter:
Whole eggs ......................................................18–24
Preparation of Homogenized Whole Egg:
Use fresh eggs, less than 1 week old. Scrub the shells
with soap. Let stand in a soap solution for 30 min.
Rinse in running water. Soak eggs in 70% ethanol for
15 min. Break the eggs into a sterile container. Ho
mogenize by shaking. Filter through four layers of
sterile cheesecloth into a sterile graduated cylinder.
Measure out 1.0L.
Preparation of Medium: Add components, ex
cept homogenized whole egg, to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Aseptically add 200.0mL of sterile ho
mogenized whole egg. Mix thoroughly. Aseptically
distribute into sterile screw-capped tubes in 7.0mL
volumes. Inspissate at 85°–90°C (moist heat) for 60
min.
Use: For the isolation and cultivation of Mycobacte
rium species, except for Mycobacterium leprae.
