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Oatmeal Agar A (ISP-3) (DSMZ Medium 609)

[所屬分類:培養基配方] [發布時間:2021-12-7] [發布人:網站管理員2] [閱讀次數:] [返回]
Oatmeal Agar A
(ISP-3)
(DSMZ Medium 609)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Oatmeal (Quaker White Oats) ..........................20.0g
Agar ..................................................................18.0g
Trace salts solution ......................................... 1.0mL
pH 7.0 ± 0.2 at 25°C
Trace Salt Solution:
Composition per 100mL:
FeSO4·7H2O........................................................0.1g
MnCl2·4H2O .......................................................0.1g
ZnSO4·7H2O .......................................................0.1g
Preparation of Trace Salt Solution: Add com
ponents to 100.0mL distilled/deionized water. Mix
thoroughly.
Preparation of Medium: Add oats to 1.0L dis
tilled/deionized water. Adjust pH to 7.2. Gently heat
and bring to boiling. Cook or steam for 20 min. Filter
through cheese cloth. Add 18.0g agar. Add 1.0mL
trace salt solution. Bring volume to 1.0L distilled/
deionized water. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Pour
into Petri dishes or distribute to sterile tubes.
Use: For the cultivation of various fungi.
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