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NZC Broth

[所屬分類:培養基配方] [發布時間:2021-12-7] [發布人:網站管理員2] [閱讀次數:] [返回]
NZC Broth

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

N-Z-Amine A™................................................10.0g
NaCl....................................................................5.0g
MgCl2·6H2O .......................................................2.0g
Casamino acids solution ................................. 5.0mL
Casamino Acids Solution:
Composition per 10.0mL:
Casamino acids ...................................................2.0g
Preparation of Casamino Acids Solution:
Add casamino acids to distilled/deionized water and
bring volume to 10.0mL. Mix thoroughly. Filter ster
ilize.
Preparation of Medium: Add components, ex
cept casamino acids solution, to distilled/deionized
water and bring volume to 990.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 5.0mL of sterile casamino acids so
lution. Mix thoroughly. Aseptically distribute into
sterile tubes or flasks.
Use: For the cultivation of Escherichia coli.
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