培養基配方

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Novobiocin Agar

[所屬分類:培養基配方] [發布時間:2021-12-6] [發布人:網站管理員2] [閱讀次數:] [返回]

Novobiocin Agar

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................15.0g

Peptone ...............................................................5.0g

NaCl....................................................................5.0g

Yeast extract........................................................2.0g

Beef extract.........................................................1.0g

Novobiocin solution...................................... 10.0mL

Novobiocin Solution:

Composition per 10.0mL:

Novobiocin ....................................................10.0mg

Preparation of Novobiocin Solution: Add no

vobiocin to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept novobiocin solution, to distilled/deionized water

and bring volume to 990.0mL. Mix thoroughly. Gen

tly heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Aseptically add 10.0mL of

sterile novobiocin solution. Mix thoroughly. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Staphylococcus aureus.