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Nitrate Reduction Broth

[所屬分類:培養基配方] [發布時間:2021-12-6] [發布人:網站管理員2] [閱讀次數:] [返回]
Nitrate Reduction Broth

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................13.0g
NaCl....................................................................5.0g
Yeast extract........................................................5.0g
Heart muscle, solids from infusion.....................2.0g
KNO3 or NaNO3 .................................................2.0g
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Dispense in 3.0mL
amounts into screw-capped tubes, add inverted vials,
and autoclave at 121°C for 15 min.
Use: For the differentiation of a variety of nonfer
menting Gram-negative bacteria based on their abili
ty to reduce nitrate to nitrite or form N2 gas. Test for
nitrates with sulfanilic acid and α-naphthylamine re
agents. Bacteria that reduce nitrate to nitrite turn the
reagents red or pink.
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