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培養基配方
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Nitrate Broth, Enriched
[所屬分類:培養基配方] [發布時間:2021-12-6] [發布人:網站管理員2] [閱讀次數:] [返回]
Nitrate Broth, Enriched
山東拓普生物工程有限公司 培養基配方 //ljxinglong.com
Composition per liter:
Pancreatic digest of casein................................13.0g
NaCl....................................................................5.0g
Yeast extract........................................................5.0g
Heart muscle, solids from infusion.....................2.0g
KNO3 ..................................................................2.0g
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Distribute into test tubes that con
tain an inverted Durham tube. Autoclave for 15 min
at 15 psi pressure–121°C.
Use: For the differentiation of aerobic and faculta
tive Gram-negative microorganisms based on their
ability to reduce nitrate to nitrite or form N2 gas. Test
for nitrates with sulfanilic acid and α-naphthylamine
reagents. Bacteria that reduce nitrate to nitrite turn
the reagents red or pink.



