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培養基配方
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Nitrate Agar
[所屬分類:培養基配方] [發布時間:2021-12-6] [發布人:網站管理員2] [閱讀次數:] [返回]
Nitrate Agar
山東拓普生物工程有限公司 培養基配方 //ljxinglong.com
Composition per liter:
Agar ..................................................................12.0g
Peptone................................................................5.0g
Beef extract.........................................................3.0g
KNO3 ..................................................................1.0g
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes. Autoclave for 15 min at 15 psi
pressure–121°C. Allow tubes to cool in a slanted po
sition.
Use: For the differentiation of aerobic and faculta
tive Gram-negative microorganisms based on their
ability to reduce nitrate. Test for nitrates with sulfa
nilic acid and α-naphthylamine reagents. Bacteria
that reduce nitrate to nitrite turn the reagents red or
pink.
