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培養基配方

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Mycobactosel™ Agar

[所屬分類:培養基配方] [發布時間:2021-12-6] [發布人:網站管理員2] [閱讀次數:] [返回]

Mycobactosel™ Agar

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................13.5g

Bovine albumin fraction V .................................5.0g

Glucose ...............................................................2.0g

Na2HPO4 .............................................................1.5g

KH2PO4...............................................................1.5g

Pancreatic digest of casein..................................1.0g

NaCl..................................................................0.85g

(NH4)2SO4...........................................................0.5g

Monosodium glutamate ......................................0.5g

Sodium citrate.....................................................0.4g

MgSO4·7H2O ....................................................0.05g

Ferric ammonium citrate...................................0.04g

Pyridoxine........................................................1.0mg

ZnSO4·7H2O ....................................................1.0mg

CuSO4·5H2O ....................................................1.0mg

Biotin ...............................................................0.5mg

CaCl2·H2O........................................................0.5mg

Malachite Green.............................................0.25mg

Antibiotic solution ........................................ 10.0mL

Catalase solution........................................... 10.0mL

Glycerol .......................................................... 5.0mL

Oleic acid ...................................................... 0.06mL

pH 6.6 ± 0.2 at 25°C

Source: This medium is available as a prepared me

dium from BD Diagnostic Systems.

Antibiotic Solution:

Composition per 10.0mL:

Cycloheximide..................................................0.36g

Nalidixic acid....................................................0.02g

Lincomycin......................................................2.0mg

Preparation of Antibiotic Solution: Add com

ponents to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Catalase Solution:

Composition per 10.0mL:

Catalase............................................................3.0mg

Preparation of Catalase Solution: Add catalase

to distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept antibiotic solution and catalase solution, to dis

tilled/deionized water and bring volume to 980.0mL.

Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 45°–50°C. Aseptically add sterile antibiotic solu

tion and sterile catalase solution. Mix thoroughly.

Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: For the selective isolation of mycobacteria

from specimens containing mixed flora.

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Mycobactosel™ Agar