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培養基配方

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Mueller-Hinton Agar with Horse Blood (LMG Medium 49)

[所屬分類:培養基配方] [發布時間:2021-12-6] [發布人:網站管理員2] [閱讀次數:] [返回]

Mueller-Hinton Agar with Horse Blood

(LMG Medium 49)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Beef infusion from..........................................300.0g

Acid hydrolysate of casein................................17.5g

Agar ..................................................................17.0g

Starch ..................................................................1.5g

Horse blood, sterile defribinated................... 50.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium without horse blood is avail

able as a premixed powder from BD Diagnostic Sys

tems and Oxoid.

Preparation of Medium: Add components, ex

cept horse blood, to 950.0mL distilled/deionized wa

ter and bring volume to 1.0L. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically

add 50.0mL sterile horse blood. Mix thoroughly. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Campy

lobacter spp. and other bacteria.