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MRS with Fermented Wort

[所屬分類:培養基配方] [發布時間:2021-12-3] [發布人:網站管理員2] [閱讀次數:] [返回]
MRS with Fermented Wort

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Solution A................................................... 800.0mL
Solution B ................................................... 200.0mL
pH 6.2 ± 0.2 at 25°C
Solution A:
Composition per liter:
Glucose .............................................................20.0g
Peptone..............................................................10.0g
Agar ..................................................................10.0g
Beef extract.........................................................8.0g
Sodium acetate·3H2O..........................................5.0g
Yeast extract........................................................4.0g
K2HPO4...............................................................2.0g
Triammonium citrate ..........................................2.0g
MgSO4·7H2O ......................................................0.2g
MnSO4·4H2O ....................................................0.05g
Sorbitan monooleate ....................................... 1.0mL
Preparation of Solution A: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 50°–55°C.
Solution B:
Composition per liter:
Malt extract.......................................................15.0g
Maltose............................................................12.75g
Dextrin ..............................................................2.75g
Glycerol ............................................................2.35g
K2HPO4...............................................................1.0g
NH4Cl .................................................................1.0g
Pancreatic digest of gelatin...............................0.78g
Preparation of Solution B: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool
to room temperature. Inoculate with Saccharomyces
cerevisiae to allow wort to ferment. Incubate for 24–
48 hr. Allow to cool to room temperature. Filter
through Whatman filter paper to remove solids. Filter
sterilize.
Preparation of Medium: Aseptically combine
800.0mL of sterile solution A and 200.0mL of sterile
solution B. Mix thoroughly. Aseptically distribute
into sterile tubes or flasks.
Use: For the cultivation of yeasts and fungi.
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