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培養基配方

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MRS Broth (DeMan, Rogosa, Sharpe Broth)

[所屬分類:培養基配方] [發布時間:2021-12-3] [發布人:網站管理員2] [閱讀次數:] [返回]

MRS Broth

(DeMan, Rogosa, Sharpe Broth)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Glucose .............................................................20.0g

Peptone..............................................................10.0g

Beef extract.........................................................8.0g

Sodium acetate·3H2O..........................................5.0g

Yeast extract........................................................4.0g

K2HPO4...............................................................2.0g

Triammonium citrate ..........................................2.0g

MgSO4·7H2O ......................................................0.2g

MnSO4·4H2O ....................................................0.05g

Sorbitan monooleate ....................................... 1.0mL

pH 6.2 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C.

Use: For the cultivation of lactic acid bacteria.

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MRS Broth (DeMan, Rogosa, Sharpe Broth)