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MRS Agar with Cysteine (LMG Medium 118)

[所屬分類:培養基配方] [發布時間:2021-12-3] [發布人:網站管理員2] [閱讀次數:] [返回]
MRS Agar with Cysteine
(LMG Medium 118)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Glucose .............................................................20.0g
Peptone..............................................................10.0g
Agar ..................................................................12.0g
Beef extract.........................................................5.0g
Sodium acetate·3H2O..........................................5.0g
Yeast extract........................................................5.0g
KH2PO4...............................................................2.0g
Diammonium hydrogen citrate ...........................2.0g
MgSO4·7H2O ......................................................0.2g
MnSO4·4H2O .................................................38.0mg
L-Cysteine solution ....................................... 10.0mL
Tween 80......................................................... 1.0mL
pH 6.5 ± 0.2 at 25°C
L-Cysteine Solution:
Composition per 10.0mL:
L-Cysteine ...........................................................0.5g
Preparation of L-Cysteine Solution: Add 0.1g
of L-cysteine to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept L-cysteine solution, to distilled/deionized water
and bring volume to 990.0mL. Mix thoroughly. Ad
just pH to 6.5. Gently heat and bring to boiling. Dis
tribute into tubes or flasks. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 50°C. Aseptically
add 10.0mL of sterile L-cysteine solution. Mix thor
oughly. Aseptically pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the isolation and cultivation of Leuconos
toc mesenteroides and other lactic acid bacteria.
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