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MP 5 Medium (Mineral Pectin 5 Medium)

[所屬分類:培養基配方] [發布時間:2021-12-3] [發布人:網站管理員2] [閱讀次數:] [返回]
MP 5 Medium
(Mineral Pectin 5 Medium)


山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar solution .............................................. 500.0mL
Basal medium ............................................. 250.0mL
Mineral solution.......................................... 250.0mL
pH 5.0–6.0 at 25°C
Agar Solution:
Composition per 500.0mL:
Agar ..................................................................15.0g
Preparation of Agar Solution: Add agar to dis
tilled/deionized water and bring volume to 500.0mL.
Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Basal Medium:
Composition per 250.0mL:
Na2HPO4.............................................................6.0g
Pectin, citrus or apple .........................................5.0g
KH2PO4...............................................................4.0g
NH4SO4...............................................................2.0g
Yeast extract........................................................1.0g
Preparation of Basal Medium: Add compo
nents to distilled/deionized water and bring volume
to 250.0mL. Mix thoroughly. Gently heat and bring
to boiling.
Mineral Solution:
Composition per 250.0mL:
FeSO4 (0.1% solution).................................... 1.0mL
MgSO4·7H2O (20% solution) ......................... 1.0mL
CaCl2·2H2O (0.1% solution)........................... 1.0mL
H3BO3 (0.001% solution) ............................... 1.0mL
MnSO4·H2O (0.001% solution) ...................... 1.0mL
ZnSO4·7H2O (0.007% solution....................... 1.0mL
CuSO4·5H2O (0.005% solution) ..................... 1.0mL
MoO3 (0.001% solution)................................. 1.0mL
Preparation of Mineral Solution: Add compo
nents to distilled/deionized water and bring volume
to 250.0mL. Mix thoroughly.
Preparation of Medium: Combine 250.0mL of
basal medium and 250.0mL of mineral solution. Mix
thoroughly. Adjust pH to 5.0–6.0 with 1N HCl. Auto
clave the basal medium-mineral solution and agar solu
tion separately for 15 min at 15 psi pressure–121°C.
Cool to 45°–50°C. Aseptically combine the two sterile
solutions. Mix thoroughly. Pour immediately into ster
ile Petri dishes to prevent hydrolysis of the agar.
Use: For the cultivation of microorganisms that pro
duce polygalactanase.
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