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Motility Nitrate Medium, Buffered (BAM M102)

[所屬分類:培養基配方] [發布時間:2021-12-2] [發布人:網站管理員2] [閱讀次數:] [返回]
Motility Nitrate Medium, Buffered
(BAM M102)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Peptone................................................................5.0g
Galactose.............................................................5.0g
Agar ....................................................................3.0g
Beef extract.........................................................3.0g
Na2HPO4 .............................................................2.5g
KNO3 ..................................................................1.0g
Glycerin .......................................................... 5.0mL
pH 7.3 ± 0.1 at 25°C
Preparation of Medium: Add components, ex
cept agar, to distilled/deionized water and bring vol
ume to 1.0L. Mix thoroughly. Gently heat until
dissolved. Add agar. Gently heat until boiling. Dis
tribute into tubes in 11.0mL volumes. Autoclave for
15 min at 15 psi pressure–121°C. If not used within
4 hr, heat tubes to 100°C for 10 min.
Use: For the cultivation and observation of the
motility of Clostridium perfringens.
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