山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Antibiotic Assay Medium No. 20
(Yeast Beef Broth)
Composition per liter:
Peptone........................................................................................15.0g
Glucose .......................................................................................11.0g
Yeast extract..................................................................................6.5g
K 2 HPO 4 .......................................................................................3.68g
NaCl..............................................................................................3.5g
Beef extract...................................................................................1.5g
KH 2 PO 4 .......................................................................................1.32g
pH 6.6 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C.
Use: For assaying the mycostatic activity of pharmaceutical prepara-
tions.