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Middlebrook 7H10 Agar with Middle brook OADC Enrichment and Hemin (Hemin Medium for Mycobacterium)

[所屬分類:培養基配方] [發布時間:2021-12-2] [發布人:網站管理員2] [閱讀次數:] [返回]
Middlebrook 7H10 Agar with Middle
brook OADC Enrichment and Hemin
(Hemin Medium for Mycobacterium)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................15.0g
Na2HPO4.............................................................1.5g
KH2PO4...............................................................1.5g
(NH4)2SO4 ..........................................................0.5g
L-Glutamic acid...................................................0.5g
Sodium citrate.....................................................0.4g
Ferric ammonium citrate...................................0.04g
MgSO4·7H2O..................................................0.025g
ZnSO4·7H2O ....................................................1.0mg
CuSO4·5H2O....................................................1.0mg
Pyridoxine........................................................1.0mg
Biotin ...............................................................0.5mg
CaCl2·2H2O .....................................................0.5mg
Malachite Green.............................................0.25mg
Middlebrook OADC enrichment ................ 100.0mL
Glycerol .......................................................... 5.0mL
Hemin solution................................................ 3.9mL
pH 6.6 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Middlebrook OADC Enrichment:
Composition per 100.0mL:
Bovine albumin fraction V .................................5.0g
Glucose ...............................................................2.0g
NaCl..................................................................0.85g
Oleic acid..........................................................0.05g
Catalase............................................................4.0mg
Preparation of Middlebrook OADC Enrich
ment: Add components to distilled/deionized water
and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Hemin Solution:
Composition per 100.0mL:
Hemin .................................................................1.0g
NaOH (1N solution)...................................... 20.0mL
Preparation of Hemin Solution: Add hemin to
20.0mL of 1N NaOH solution. Mix thoroughly. Bring
volume to 100.0mL with distilled/deionized water.
Preparation of Medium: Add glycerol to
891.1mL of distilled/deionized water and add re
maining components, except Middlebrook OADC
enrichment. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°–55°C. Aseptically add 100.0mL
of sterile Middlebrook OADC enrichment. Mix thor
oughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the isolation, cultivation, and maintenance
of Mycobacterium species, including Mycobacte
rium tuberculosis. For the cultivation and mainte
nance of Mycobacterium haemophilum. Also used
for determining the antimicrobial susceptibility of
mycobacteria.
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