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培養基配方

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Middlebrook 7H10 Agar with Middlebrook ADC Enrichment

[所屬分類:培養基配方] [發布時間:2021-12-2] [發布人:網站管理員2] [閱讀次數:] [返回]

Middlebrook 7H10 Agar with

Middlebrook ADC Enrichment

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................15.0g

Na2HPO4.............................................................1.5g

KH2PO4...............................................................1.5g

(NH4)2SO4 ..........................................................0.5g

L-Glutamic acid...................................................0.5g

Sodium citrate.....................................................0.4g

Ferric ammonium citrate...................................0.04g

MgSO4·7H2O..................................................0.025g

ZnSO4·7H2O ....................................................1.0mg

CuSO4·5H2O....................................................1.0mg

Pyridoxine........................................................1.0mg

Biotin ...............................................................0.5mg

CaCl2·2H2O .....................................................0.5mg

Malachite Green.............................................0.25mg

Middlebrook ADC enrichment................... 100.0mL

Glycerol .......................................................... 5.0mL

pH 6.6 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Middlebrook ADC Enrichment:

Composition per 100.0mL:

Bovine albumin fraction V .................................5.0g

Glucose ...............................................................2.0g

Catalase...........................................................0.003g

Distilled water............................................. 100.0mL

Source: This enrichment is available as a prepared

enrichment from BD Diagnostic Systems.

Preparation of Middlebrook ADC Enrich

ment: Add components to distilled/deionized water

and bring volume to 100.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add glycerol to

900.0mL of distilled/deionized water and add re

maining components, except Middlebrook ADC en

richment. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°–55°C. Aseptically add 100.0mL

of sterile Middlebrook ADC enrichment. Mix thor

oughly. Pour into sterile Petri dishes or distribute into

sterile tubes.

Use: For the isolation, cultivation, and maintenance

of Mycobacterium species, including Mycobacte

rium tuberculosis. Also used for determining the

antimicrobial susceptibility of mycobacteria.

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Middlebrook 7H10 Agar with Middlebrook ADC Enrichment