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Middlebrook 13A Medium

[所屬分類:培養基配方] [發布時間:2021-12-1] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per 112.5mL:

Casein hydrolysate..............................................0.1g

Tween 80...........................................................0.02g

Sodium polyanetholesulfonate........................0.025g

Catalase........................................................36,000U

14C-substrate ................................. 125μCi (185kBq)

Middlebrook 7H9 broth .............................. 100.0mL

Middlebrook 13A enrichment....................... 12.5mL

pH 6.6 ± 0.2 at 25°C

Middlebrook 7H9 Broth:

Composition per liter:

Na2HPO4.............................................................2.5g

KH2PO4...............................................................1.0g

Monosodium glutamate ......................................0.5g

(NH4)2SO4 ..........................................................0.5g

Sodium citrate.....................................................0.1g

MgSO4·7H2O....................................................0.05g

Ferric ammonium citrate...................................0.04g

CuSO4·5H2O....................................................1.0mg

Pyridoxine........................................................1.0mg

ZnSO4·7H2O ....................................................1.0mg

Biotin ...............................................................0.5mg

CaCl2·2H2O .....................................................0.5mg

Glycerol .......................................................... 2.0mL

Preparation of Middlebrook 7H9 Broth: Add

components to distilled/deionized water and bring

volume to 1.0L. Mix thoroughly.

Middlebrook 13A Enrichment:

Composition per 20.0mL:

Bovine serum albumin........................................3.0g

Preparation of Middlebrook 13A Enrich

ment: Add bovine serum albumin to distilled/deion

ized water and bring volume to 20.0mL. Mix

thoroughly. Filter sterilize.

Preparation of Medium: To 100.0mL of Middle

brook 7H9 broth, add remaining components, except

Middlebrook 13A enrichment. Mix thoroughly. Fil

ter sterilize. Aseptically distribute into bottles in

4.0mL volumes. Prior to inoculation, aseptically add

0.5mL of Middlebrook 13A enrichment to each bot

tle. Mix thoroughly.

Use: For the cultivation of Mycobacterium species

from the blood of patients suspected of having myco

bacteremia.

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Middlebrook 13A Medium