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培養基配方

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Membrane Clostridium perfringens Medium (m-CP Medium)

[所屬分類:培養基配方] [發布時間:2021-12-1] [發布人:網站管理員2] [閱讀次數:] [返回]

Membrane Clostridium perfringens

Medium

(m-CP Medium)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per 1040mL:

Tryptose ............................................................30.0g

Yeast extract......................................................20.0g

Agar ..................................................................15.0g

Sucrose................................................................5.0g

L-Cysteine·HCl·H2O ...........................................1.0g

MgSO4·7H2O ......................................................0.1g

Bromcresol Purple ............................................0.04g

Phenolphthalein solution............................... 20.0mL

Indoxyl-β-D-glucoside solution ...................... 8.0mL

Selective supplement solution ........................ 8.0mL

Ferric chloride solution................................... 4.0mL

pH 7.6 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Selective Supplement Solution:

Composition per 8.0mL:

D-Cycloserine......................................................0.8g

Polymyxin B sulfate ......................................50.0mg

Preparation of Selective Supplement Solu

tion: Add componetns to distilled/deionized water

and bring volume to 8.0mL. Mix thoroughly. Filter

sterilize.

Indoxyl-β-D-glucoside Solution:

Composition per 10.0mL:

Indoxyl-β-D-glucoside ...................................75.0mg

Preparation of Indoxyl-β-D-glucoside Solu

tion: Add indoxyl-β-D-glucoside to distilled/deion

ized water and bring volume to 10.0mL. Mix

thoroughly. Filter sterilize.

Ferric Chloride Solution:

Composition per 10.0mL:

FeCl3·6H2O.......................................................0.45g

Preparation of Ferric Chloride Solution: Add

ferric chloride to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Phenolphthalein Solution:

Composition per 20.0mL:

Phenolphthalein biphosphate

tetrasodium salt ..........................................0.15g

Preparation of Phenolphthalein Solution:

Add phenolphthalein biphosphate tetrasodium salt to

distilled/deionized water and bring volume to

20.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept selective supplement solution, phenolophthalein

solution, ferric chloride solution, and indoxyl-β-D

glucoside to distilled/deionized water and bring vol

ume to 1.0L. Mix thoroughly. Distribute into tubes or

flasks. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°C. Aseptically add 8.0mL selec

tive supplement solution, 20.0mL phenolophthalein

solution, 4.0mL ferric chloride solution, and 8.0mL

indoxyl-β-D-glucoside. Mix thoroughly. Pour into

sterile Petri dishes or aseptically distribute into ster

ile tubes.

Use: For the rapid isolation and presumptive identi

fication of Clostridium perfringens from food and

water samples. A selective and chromogenic medium

for the presumptive identification of Clostridium

perfringens, especially from water samples. Recom

mended in European Council Directive 98/83/EC for

testing the quality of water intended for human con

sumption.C. perfringens colonies have a characteris

tic opaque yellow appearance. Most other Clostridi

um spp. will appear as either purple colonies, due to

the lack of sucrose fermentation, or blue/green colo-

nies where the organism is still cleaving indoxyl-β-

D-glucoside and also fermenting sucrose.

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Membrane Clostridium perfringens Medium (m-CP Medium)