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Medium N

[所屬分類:培養基配方] [發布時間:2021-12-1] [發布人:網站管理員2] [閱讀次數:] [返回]
Medium N

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................20.0g
Glucose .............................................................20.0g
Yeast nitrogen base without amino acids............6.7g
Casamino acids, vitamin free..............................2.0g
Isoleucine............................................................0.1g
Valine ..................................................................0.1g
Deoxythymidine-5′-monophosphate
solution ................................................... 10.0mL
Deoxythymidine-5′-Monophosphate Solution:
Composition per 10mL:
Deoxythymidine-5′-monophosphate .............15.0mg
Preparation of Deoxythymidine-5′-Monophos
phate Solution: Add deoxythymidine-5′-monophos
phate to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept deoxythymidine-5′-monophosphate solution, to
distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 60°C. Aseptically add 10.0mL of
sterile deoxythymidine-5′-monophosphate solution.
Mix thoroughly. Pour into sterile Petri dishes or dis
tribute into sterile tubes.
Use: For the cultivation and maintenance of Saccha
romyces cerevisiae.
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