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MD Medium

[所屬分類:培養基配方] [發布時間:2021-12-1] [發布人:網站管理員2] [閱讀次數:] [返回]
MD Medium

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................20.0g
L-Malic acid ......................................................20.0g
Pancreatic digest of casein................................10.0g
D-Glucose............................................................5.0g
Casamino acids ...................................................3.0g
Pancreatic digest of soybean meal......................1.5g
Tween 80.............................................................1.0g
Yeast extract........................................................1.0g
Bromcresol Green solution ........................... 20.0mL
pH 7.0 ± 0.2 at 25°C
Bromcresol Green Solution:
Composition per 30.0mL:
Bromcresol Green ...............................................0.1g
NaOH (0.01N solution)................................. 30.0mL
Preparation of Bromcresol Green Solution:
Add Bromcresol Green to 30.0mL of NaOH solution.
Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Adjust pH to 7.0 with
10N KOH. Autoclave for 15 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the isolation and cultivation of Salmonella
species from foods.
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