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McClung-Toabe Egg Yolk Agar, CDC Modified (CDC Modified McClung Toabe Egg Yolk Agar)

[所屬分類:培養基配方] [發布時間:2021-12-1] [發布人:網站管理員2] [閱讀次數:] [返回]

McClung-Toabe Egg Yolk Agar,

CDC Modified

(CDC Modified McClung

Toabe Egg Yolk Agar)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................40.0g

Agar ..................................................................25.0g

Na2HPO4.............................................................5.0g

Yeast extract........................................................5.0g

Glucose ...............................................................2.0g

NaCl....................................................................2.0g

KH2PO4...............................................................1.0g

Egg yolk emulsion, 50%............................. 100.0mL

MgSO4·7H2O (5% solution) ........................... 0.2mL

pH 7.3 ± 0.2 at 25°C

Egg Yolk Emulsion, 50%:

Composition per 100.0mL:

Chicken egg yolks..................................................11

Whole chicken egg...................................................1

NaCl (0.9% solution) .................................... 50.0mL

Preparation of Egg Yolk Emulsion, 50%:

Soak eggs with 1:100 dilution of saturated mercuric

chloride solution for 1 min. Crack eggs and separate

yolks from whites. Mix egg yolks with 1 chicken egg.

Measure 50.0mL of egg yolk emulsion and add to

50.0mL of 0.9% NaCl solution. Mix thoroughly. Fil

ter sterilize. Warm to 45°–50°C.

Preparation of Medium: Add components—ex

cept egg yolk emulsion, 50%—to distilled/deionized

water and bring volume to 900.0mL. Mix thoroughly.

Gently heat while stirring and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

50°–55°C. Aseptically add 100.0mL of sterile egg

yolk emulsion, 50%. Mix thoroughly. Pour into ster

ile Petri dishes in 15.0mL volumes.

Use: For the cultivation of a wide variety of anaero

bic bacteria. For the differentiation of anaerobic bac

teria based on lecithinase production, lipase produc

tion, and proteolytic ability. Bacteria that produce

lecithinase appear as colonies surrounded by a zone

of insoluble precipitate. Bacteria that produce lipase

appear as colonies with a pearly iridescent sheen.

Bacteria that produce proteolytic activity appear as

colonies surrounded by a clear zone.

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McClung-Toabe Egg Yolk Agar, CDC Modified (CDC Modified McClung Toabe Egg Yolk Agar)