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培養基配方

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McClung-Toabe Agar, Modified

[所屬分類:培養基配方] [發布時間:2021-12-1] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Proteose peptone No. 2 .....................................20.0g

Agar ..................................................................20.0g

Yeast extract........................................................5.0g

Pancreatic digest of casein..................................5.0g

NaCl....................................................................5.0g

Sodium thioglycolate ..........................................1.0g

Egg yolk emulsion, 50%............................... 80.0mL

pH 7.6 ± 0.2 at 25°C

Egg Yolk Emulsion, 50%:

Composition per 100.0mL:

Chicken egg yolks..................................................11

Whole chicken egg...................................................1

NaCl (0.9% solution) .................................... 50.0mL

Preparation of Egg Yolk Emulsion, 50%:

Soak eggs with 1:100 dilution of saturated mercuric

chloride solution for 1 min. Crack eggs and separate

yolks from whites. Mix egg yolks with 1 chicken egg.

Measure 50.0mL of egg yolk emulsion and add to

50.0mL of 0.9% NaCl solution. Mix thoroughly. Fil

ter sterilize. Warm to 45°–50°C.

Preparation of Medium: Add components, ex

cept egg yolk emulsion, 50%, to distilled/deionized

water and bring volume to 920.0mL. Mix thoroughly.

Gently heat while stirring and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

50°–55°C. Aseptically add 80.0mL of sterile egg

yolk emulsion, 50%. Mix thoroughly. Pour into ster

ile Petri dishes in 20.0mL volumes.

Use: For the cultivation of Clostridium botulinum.

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