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培養基配方

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McClung-Toabe Agar, Modified

[所屬分類:培養基配方] [發布時間:2021-12-1] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Proteose peptone No. 2.....................................40.0g

Agar ..................................................................20.0g

Na2HPO4.............................................................5.0g

Glucose ...............................................................2.0g

NaCl....................................................................2.0g

KH2PO4...............................................................1.0g

MgSO4·7H2O......................................................0.1g

Neomycin............................................................0.1g

Egg yolk emulsion, 50%............................. 100.0mL

Hemin solution................................................ 1.0mL

pH 7.6 ± 0.2 at 25°C

Egg Yolk Emulsion, 50%:

Composition per 100.0mL:

Chicken egg yolks..................................................11

Whole chicken egg ..................................................1

NaCl (0.9% solution).................................... 50.0mL

Preparation of Egg Yolk Emulsion, 50%:

Soak eggs with 1:100 dilution of saturated mercuric

chloride solution for 1 min. Crack eggs and separate

yolks from whites. Mix egg yolks with 1 chicken egg.

Measure 50.0mL of egg yolk emulsion and add to

50.0mL of 0.9% NaCl solution. Mix thoroughly. Fil

ter sterilize. Warm to 45°–50°C.

Hemin Solution:

Composition per 100.0mL:

Hemin .................................................................0.5g

NaOH (1N solution)...................................... 20.0mL

Preparation of Hemin Solution: Add hemin to

20.0mL of 1N NaOH solution. Mix thoroughly.

Bring volume to 100.0mL with distilled/deionized

water.

Preparation of Medium: Add components, ex

cept egg yolk emulsion, 50%, to distilled/deionized

water and bring volume to 900.0mL. Mix thoroughly.

Gently heat while stirring and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

50°–55°C. Aseptically add 100.0mL of sterile egg

yolk emulsion, 50%. Mix thoroughly. Pour into ster

ile Petri dishes in 20.0mL volumes.

Use: For the cultivation of Clostridium species. For

the differentiation of Clostridium species based on

lecithinase production and lipase production. Bacte

ria that produce lecithinase appear as colonies sur

rounded by a zone of insoluble precipitate. Bacteria

that produce lipase appear as colonies with a pearly

iridescent sheen.

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McClung-Toabe Agar, Modified