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培養基配方

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Mannitol Yolk Polymyxin Agar (MYP Agar)

[所屬分類:培養基配方] [發布時間:2021-12-1] [發布人:網站管理員2] [閱讀次數:] [返回]

Mannitol Yolk Polymyxin Agar

(MYP Agar)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per 110.0mL:

Agar ....................................................................1.5g

NaCl....................................................................1.0g

Peptone ...............................................................1.0g

D-Mannitol ..........................................................1.0g

(NH4)2PO4 ..........................................................0.1g

Meat extract ........................................................0.1g

Phenol Red.......................................................2.5mg

Egg yolk emulsion, 20%............................... 10.0mL

Polymyxin B solution ..................................... 1.0mL

pH 7.1 ± 0.2 at 25°C

Egg Yolk Emulsion, 20%:

Composition per 100.0mL:

Chicken egg yolks..................................................11

Whole chicken egg ..................................................1

NaCl (0.9% solution).................................... 80.0mL

Preparation of Egg Yolk Emulsion, 20%:

Soak eggs with 1:100 dilution of saturated mercuric

chloride solution for 1 min. Crack eggs and separate

yolks from whites. Mix egg yolks with 1 chicken egg.

Measure 20.0mL of egg yolk emulsion and add to

80.0mL of 0.9% NaCl solution. Mix thoroughly. Fil

ter sterilize. Warm to 45°–50°C.

Polymyxin B Solution:

Composition per 1.0mL:

Polymyxin B ....................................................1.0mg

Preparation of Polymyxin B Solution: Add

polymyxin B to distilled/deionized water and bring

volume to 1.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components—ex

cept egg yolk emulsion, 20%, and polymyxin B solu

tion—to distilled/deionized water and bring volume

to 100.0mL. Mix thoroughly. Gently heat and bring

to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add 10.0mL

of sterile egg yolk emulsion, 20%, and 1.0mL of ster

ile polymyxin B solution. Mix thoroughly. Pour into

sterile Petri dishes.

Use: For the cultivation and maintenance of Bacillus

cereus.

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Mannitol Yolk Polymyxin Agar (MYP Agar)