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山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Antibiotic Assay Medium No. 10
(Polymyxin Seed Agar)
Composition per liter:
Casein enzymatic hydrolysate ....................................................17.0g
Agar ............................................................................................12.0g
NaCl..............................................................................................5.0g
Papaic digest of soybean meal......................................................3.0g
K2HPO 4 .........................................................................................2.5g
Glucose .........................................................................................2.5g
pH 7.2 ± 0.2 at 25°C
Source: This medium, without polysorbate 80, is available as a pre-
mixed powder from HiMedia.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes.
Use: For antibiotic assay testing. For seed agar for the plate assay of
carbenicillin, colistimethate, and polymyxin B.