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Mannitol Maltose Agar (BAM M96)

[所屬分類:培養基配方] [發布時間:2021-12-1] [發布人:網站管理員2] [閱讀次數:] [返回]
Mannitol Maltose Agar
(BAM M96)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

NaCl..................................................................20.0g
Agar ..................................................................13.0g
D-Mannitol ........................................................10.0g
Maltose .............................................................10.0g
Beef extract.........................................................5.0g
Papaic digest of soybean meal............................5.0g
Polypeptone ........................................................5.0g
Dye stock solution, 1000× .............................. 1.0mL
pH 7.8 ± 0.2 at 25°C
Dye Stock Solution, 1000X:
Bromthymol Blue ...............................................4.0g
Cresol Red ..........................................................4.0g
Ethanol, 95%............................................... 100.0mL
Preparation of Dye Stock Solution, 1000X:
Add Bromthymol Blue and Cresol Red to 100.0mL
of ethanol. Mix thoroughly.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Adjust to pH 7.8. Gently heat and
bring to boiling. Distribute into tubes or flasks. Auto
clave for 15 min at 15 psi pressure–121°C. Pour into
sterile Petri dishes or leave in tubes.
Use: For the cultivation of Vibrio species from
foods.
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