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Malonate Broth

[所屬分類:培養基配方] [發布時間:2021-11-30] [發布人:網站管理員2] [閱讀次數:] [返回]
Malonate Broth

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Sodium malonate ................................................3.0g
NaCl....................................................................2.0g
(NH4)2SO4...........................................................2.0g
K2HPO4...............................................................0.6g
KH2PO4...............................................................0.4g
Bromthymol Blue ...........................................0.025g
pH 6.7 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Distribute into tubes or flasks. Auto
clave for 15 min at 15 psi pressure–121°C. Avoid in
troduction of carbon and nitrogen from other sources.
Use: For the cultivation and differentiation of
coliforms and other enteric organisms, particularly
Enterobacter and Escherichia, based on their ability
to utilize malonate as the sole carbon source and
ammonium sulfate as the sole nitrogen source. Mal
onate-utilizing organisms turn the medium blue.
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