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MacConkey Agar without Salt

[所屬分類:培養基配方] [發布時間:2021-11-30] [發布人:網站管理員2] [閱讀次數:] [返回]
MacConkey Agar without Salt

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Peptone .............................................................20.0g
Agar ..................................................................12.0g
Lactose..............................................................10.0g
Bile salts..............................................................5.0g
Neutral Red.....................................................0.075g
pH 7.4 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems and Oxoid.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat while stirring until boil
ing. Autoclave for 15 min at 15 psi pressure–121°C.
Pour into sterile Petri dishes or distribute into sterile
tubes. Dry the surface of plates before inoculation.
Use: For the isolation and detection of coliforms and
enteric pathogens from urine. Provides a low electro
lyte medium on which most Proteus species will not
swarm and therefore avoids overgrowth of the plate.
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