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MacConkey Agar No. 3

[所屬分類:培養基配方] [發布時間:2021-11-30] [發布人:網站管理員2] [閱讀次數:] [返回]
MacConkey Agar No. 3

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Peptone..............................................................20.0g
Agar ..................................................................15.0g
Lactose..............................................................10.0g
NaCl....................................................................5.0g
Bile salts No. 3....................................................1.5g
Neutral Red.......................................................0.03g
Crystal Violet..................................................0.001g
pH 7.1 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from Oxoid.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat while stirring until boil
ing. Autoclave for 15 min at 15 psi pressure–121°C.
Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the selective isolation, cultivation, and dif
ferentiation of enteric pathogens, especially Salmo
nella and Shigella, in clinical specimens and in
foods.
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