99精品国产成人综合_国产不卡精品视频男人的天堂_人妻av久久一区波多野结衣_亚洲乱码卡一卡二卡新区仙踪林_人妻无码av天堂二区网站_中文字幕精品av一区二区五区_国产精品国产高清国产av_亚洲欧美日韩国产综合点击进入_亚洲欧美日韩国产制服另类_亚洲精品成av人片天堂无码

展開
個人中心 我的訂單 購物車
編號：
批號：
培養基配方
您現在的位置： 網站首頁 >> 培養基配方

M9 Medium

[所屬分類:培養基配方] [發布時間:2021-11-30] [發布人:網站管理員2] [閱讀次數:] [返回]
M9 Medium

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Na2HPO4 .............................................................6.0g
KH2PO4...............................................................3.0g
NH4Cl .................................................................1.0g
NaCl....................................................................0.5g
Glucose solution ........................................... 10.0mL
MgSO4·7H2O solution .................................... 1.0mL
Thiamine·HCl solution.................................... 1.0mL
CaCl2 solution................................................. 1.0mL
pH 7.0 ± 0.2 at 25°C
Glucose Solution:
Composition per 100.0mL:
D-Glucose ..........................................................20.0g
Preparation of Glucose Solution: Add glucose
to distilled/deionized water and bring volume to
1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C.
MgSO4·7H2O Solution:
Composition per liter:
MgSO4·7H2O ..................................................246.5g
Preparation of MgSO4·7H2O Solution: Add
MgSO4·7H2O to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C.
Thiamine·HCl Solution:
Composition per 10.0mL:
Thiamine·HCl ................................................10.0mg
Preparation of Thiamine·HCl Solution: Add
thiamine·HCl to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Filter sterilize.
CaCl2 Solution:
Composition per liter:
CaCl2 solution...................................................14.7g
Preparation of CaCl2 Solution: Add CaCl2 so
lution to distilled/deionized water and bring volume
to 1.0L. Mix thoroughly. Autoclave for 15 min at 15
psi pressure–121°C.
Preparation of Medium: Add components, ex
cept MgSO4·7H2O solution, glucose solution, thia
mine·HCl solution, and CaCl2 solution, to distilled/
deionized water and bring volume to 987.0mL. Mix
thoroughly. Adjust pH to 7.0. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to room temperature.
Aseptically add sterile MgSO4·7H2O solution, sterile
glucose solution, sterile thiamine·HCl solution, and
sterile CaCl2 solution. Mix thoroughly. Distribute
into tubes or flasks.
Use: For the cultivation and maintenance of Escher
ichia coli and a variety of other bacteria.
  版權所有：山東拓普生物工程有限公司 營銷服務熱線：　郵箱：
© 2016-2020 ALL RIGHTS RESERVED.　　
購物車 去頂部