培養基配方

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M17 Broth

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M17 Broth

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Disodium β-glycerophosphate..........................19.0g

Beef extract.........................................................5.0g

Lactose................................................................5.0g

Papaic digest of soybean meal............................5.0g

Pancreatic digest of casein..................................2.5g

Peptic digest of animal tissue .............................2.5g

Yeast extract........................................................2.5g

Ascorbic acid ......................................................0.5g

MgSO4·7H2O ....................................................0.25g

pH 7.15 ± 0.05 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems and Oxoid.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of strepto

cocci and their bacteriophages. Also used for the cul

tivation and maintenance of starter cultures for

cheese and yogurt manufacture as well as detecting

streptococcal mutants that are unable to ferment lac

tose.