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Lysine Broth Falkow with NaCl (BAM M44)/(BAM M87)

[所屬分類:培養基配方] [發布時間:2021-11-29] [發布人:網站管理員2] [閱讀次數:] [返回]
Lysine Broth Falkow with NaCl
(BAM M44)/(BAM M87)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

L-Lysine ..............................................................5.0g
Peptone or gelysate.............................................5.0g
Yeast extract........................................................3.0g
Glucose ...............................................................1.0g
Bromcresol Purple ............................................0.02g
pH 6.5 ± 0.2 at 25°C
Preparation of Medium: Add components to dis
tilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Adjust pH so that it will be 6.5 ± 0.2 after
sterilization. Distribute into 16 × 150mm screw
capped tubes in 5.0mL volumes. Autoclave medium
with loosely capped tubes for 10 min at 15 psi pressure–
121°C. Screw the caps on tightly for storage and after
inoculation.
Use: For the cultivation and differentiation of Vibrio
spp. based on their ability to decarboxylate the amino
acid lysine. Bacteria that decarboxylate lysine turn
the medium turbid purple.
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