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Lysine Arginine Iron Agar (LAIA) (BAM M86)

[所屬分類:培養基配方] [發布時間:2021-11-29] [發布人:網站管理員2] [閱讀次數:] [返回]

Lysine Arginine Iron Agar

(LAIA)

(BAM M86)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................15.0g

L-Arginine .........................................................10.0g

L-Lysine.............................................................10.0g

Peptone................................................................5.0g

Yeast extract........................................................3.0g

Glucose ...............................................................1.0g

Ferric ammonium citrate.....................................0.5g

Sodium thiosulfate ............................................0.04g

Bromcresol Purple ............................................0.02g

pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Adjust pH

to 6.8. Distribute into screw-capped tubes in 5.0mL

volumes. Autoclave for 12 min at 15 psi pressure–

121°C. Allow tubes to cool in a slanted position.

Use: For the cultivation and differentiation of bacteria

based on their ability to decarboxylate lysine, decarbox

ylate arginine, and produce H2S. Bacteria that decar

boxylate lysine or arginine turn the medium purple.

Bacteria that produce H2S appear as black colonies.