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培養基配方

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LS Differential Medium (Lactobacillus Streptococcus Differential Medium)

[所屬分類:培養基配方] [發布時間:2021-11-29] [發布人:網站管理員2] [閱讀次數:] [返回]

LS Differential Medium

(Lactobacillus Streptococcus

Differential Medium)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Glucose .............................................................20.0g

Agar ..................................................................13.0g

Pancreatic digest of casein................................10.0g

Beef extract.........................................................5.0g

NaCl....................................................................5.0g

Papaic digest of soybean meal............................5.0g

Yeast extract........................................................5.0g

L-Cysteine·HC1·H2O...........................................0.3g

Skim milk solution...................................... 100.0mL

Triphenyltetrazolium chloride solution......... 10.0mL

pH 6.1 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Skim Milk Solution:

Composition per 100.0mL:

Skim milk, antibiotic free .................................10.0g

Preparation of Skim Milk Solution: Add skim

milk to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Autoclave for 5 min at 15

psi pressure–121°C. Cool to 50°C.

Triphenyltetrazolium Chloride Solution:

Composition per 10.0mL:

2,3,5-Triphenyltetrazolium chloride ...................0.2g

Preparation of Triphenyltetrazolium Chlo

ride Solution: Add triphenyltetrazolium chloride

to distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize. Warm to

50°C.

Preparation of Medium: Add components, except

skim milk solution and triphenyltetrazolium chloride

solution, to distilled/deionized water and bring volume

to 890.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add sterile skim

milk solution and sterile triphenyltetrazolium chloride

solution. Mix thoroughly. Pour into sterile Petri dishes

or distribute into sterile tubes.

Use: For the differentiation and enumeration of lacto

bacilli and streptococci in yogurt. Lactobacillus spe

cies appear as irregular, red colonies surrounded by a

white, opaque zone. Streptococcus species appear as

round, red colonies surrounded by a clear zone.

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LS Differential Medium (Lactobacillus Streptococcus Differential Medium)