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Listeria Fermentation Broth
[所屬分類:培養基配方] [發布時間:2021-11-26] [發布人:網站管理員2] [閱讀次數:] [返回]
Listeria Fermentation Broth
山東拓普生物工程有限公司 培養基配方 //ljxinglong.com
Composition per liter:
Proteose peptone No. 3 .....................................10.0g
NaCl....................................................................5.0g
Beef extract.........................................................1.0g
Bromcresol Purple ..............................................0.1g
Carbohydrate solution................................... 10.0mL
Carbohydrate Solution:
Composition per 10.0mL:
Carbohydrate.......................................................5.0g
Preparation of Carbohydrate Solution: Add
carbohydrate to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
Use glucose, salicin, rhamnose, dulcitol, or raffinose.
Preparation of Medium: Add components, ex
cept carbohydrate solution, to distilled/deionized wa
ter and bring volume to 990.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add sterile carbohydrate solution. Mix
thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use: For the cultivation and differentiation of Liste
ria species based on the fermentation of glucose, sali
cin, rhamnose, dulcitol, and raffinose.
