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Listeria Enrichment Broth I, USDA FSIS (Listeria Primary Selective Enrichment Broth, UVM I) (University of Vermont I Listeria Primary Selective Enrichment Broth)

[所屬分類:培養基配方] [發布時間:2021-11-26] [發布人:網站管理員2] [閱讀次數:] [返回]
Listeria Enrichment Broth I, USDA FSIS
(Listeria Primary Selective
Enrichment Broth, UVM I)
(University of Vermont I Listeria
Primary Selective Enrichment Broth)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

NaCl..................................................................20.0g
Na2HPO4 ...........................................................12.0g
Beef extract.........................................................5.0g
Proteose peptone.................................................5.0g
Pancreatic digest of casein..................................5.0g
Yeast extract........................................................5.0g
KH2PO4.............................................................1.35g
Esculin ................................................................1.0g
Nalidixic acid solution.................................... 1.0mL
Acriflavine solution ........................................ 1.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from Oxoid.
Nalidixic Acid Solution:
Composition per 10.0mL:
Nalidixic acid......................................................0.2g
NaOH (0.1M solution).................................. 10.0mL
Preparation of Nalidixic Acid Solution: Add
nalidixic acid to 10.0mL of NaOH solution. Mix
thoroughly. Filter sterilize.
Acriflavine Solution:
Composition per 10.0mL:
Acriflavine ........................................................0.12g
Preparation of Acriflavine Solution: Add acri
flavine to distilled/deionized water and bring volume
to 10.0mL. Mix thoroughly. Filter sterilize. Use
freshly prepared solution.
Preparation of Medium: Add components, ex
cept nalidixic acid solution and acriflavine solution,
to distilled/deionized water and bring volume to
998.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Aseptically add sterile na
lidixic acid solution. Mix thoroughly. Store at 4°C.
Immediately prior to use, aseptically add 1.0mL of
sterile acriflavine solution. Mix thoroughly. Asepti
cally distribute into sterile tubes or flasks.
Use: For the selective isolation, cultivation, and en
richment of Listeria monocytogenes from food, milk,
and dairy products.
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