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Lauryl Tryptose Mannitol Broth with Tryptophan

[所屬分類:培養基配方] [發布時間:2021-11-26] [發布人:網站管理員2] [閱讀次數:] [返回]
Lauryl Tryptose Mannitol
Broth with Tryptophan

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................20.0g
Lactose................................................................5.0g
NaCl....................................................................5.0g
K2HPO4.............................................................2.75g
KH2PO4.............................................................2.75g
Sodium lauryl sulfate..........................................0.1g
L-Tryptophan.......................................................0.2g
pH 6.8 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from Oxoid.
Preparation of Medium: Add components to dis
tilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Distribute into tubes containing an inverted
Durham tube in 10.0mL volumes. Autoclave for 10 min
at 10 psi pressure–115°C. Cool broth quickly to 25°C.
Use: For the detection of Escherichia coli.
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