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Lauryl Tryptose Broth (Lauryl Sulfate Broth) (LST Broth) (BAM M76)

[所屬分類:培養基配方] [發布時間:2021-11-26] [發布人:網站管理員2] [閱讀次數:] [返回]
Lauryl Tryptose Broth
(Lauryl Sulfate Broth)
(LST Broth)
(BAM M76)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................20.0g
Lactose................................................................5.0g
NaCl....................................................................5.0g
K2HPO4.............................................................2.75g
KH2PO4.............................................................2.75g
Sodium lauryl sulfate..........................................0.1g
pH 6.8 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Distribute into tubes containing an
inverted Durham tube in 10.0mL volumes. Auto
clave for 12 min at 15 psi pressure–121°C. Cool
broth quickly to 25°C. For testing water samples with
10.0mL volumes, prepare medium double strength.
Use: For the detection of coliform bacteria in a variety
of specimens. Also, for the enumeration of coliform
bacteria by the multiple-tube fermentation technique.

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