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培養基配方
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Lactobacillus Medium (ATCC Medium 1006)
[所屬分類:培養基配方] [發布時間:2021-11-25] [發布人:網站管理員2] [閱讀次數:] [返回]
Lactobacillus Medium
(ATCC Medium 1006)
山東拓普生物工程有限公司 培養基配方 //ljxinglong.com
Composition per liter:
Agar ..................................................................20.0g
Pancreatic digest of casein................................10.0g
Glucose ...............................................................5.0g
Yeast extract........................................................5.0g
KH2PO4...............................................................3.0g
K2HPO4...............................................................3.0g
Tryptose ..............................................................3.0g
Sodium acetate....................................................1.0g
L-Cysteine·HCl·H2O............................................0.2g
Salt solution R................................................. 5.0mL
Tween 80......................................................... 1.0mL
pH 6.3 ± 0.2 at 25°C
Salt Solution R:
Composition per 100.0mL:
MgSO4·7H2O ....................................................11.5g
MnSO4·2H2O ......................................................2.4g
FeSO4·7H2O......................................................0.68g
Preparation of Salt Solution R: Add compo
nents to distilled/deionized water and bring volume
to 100.0mL. Mix thoroughly. Store at 4°C.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dish
es or leave in tubes.
Use: For the cultivation and maintenance of Lacto
bacillus delbrueckii and Lactobacillus jensenii.
