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Lactobacillus Heteroferm Screen Broth (MRS, Modified)

[所屬分類:培養基配方] [發布時間:2021-11-25] [發布人:網站管理員2] [閱讀次數:] [返回]

Lactobacillus Heteroferm Screen Broth

(MRS, Modified)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Glucose .............................................................20.0g

Proteose peptone No. 3.....................................10.0g

Sodium acetate....................................................5.0g

Yeast extract........................................................5.0g

2-Phenylethyl alcohol .........................................3.0g

Ammonium citrate..............................................2.0g

K2HPO4...............................................................2.0g

MgSO4·7H2O......................................................0.1g

MnSO4·H2O......................................................0.05g

Bromcresol Green.............................................0.04g

Cycloheximide.................................................4.0mg

Tween 80......................................................... 1.0mL

pH 4.3 ± 0.01 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Adjust pH to 4.3 with concentrated

HCl. Distribute into test tubes that contain an invert

ed Durham tube. Autoclave for 15 min at 15 psi pres

sure–121°C.

Use: For the isolation and cultivation of Lactobacil

lus species from foods.

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