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Lactobacilli Agar, AOAC (Lactobacilli Agar, Association of Official Analytical Chemists)

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Lactobacilli Agar, AOAC

(Lactobacilli Agar, Association of

Official Analytical Chemists)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Milk, peptonized ...............................................15.0g

Agar ..................................................................10.0g

Glucose .............................................................10.0g

Yeast extract........................................................5.0g

KH2PO4...............................................................2.0g

Sorbitan monooleate complex ............................1.0g

Tomato juice ............................................... 100.0mL

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Continue boiling for 2–3 min. Distribute into tubes in

10.0mL volumes. Autoclave for 15 min at 15 psi

pressure–121°C. Allow tubes to cool in an upright

position.

Use: For the cultivation and maintenance of stock

cultures of Lactobacillus casei ATCC 7469, Lacto

bacillus fermentum ATCC 9338, Lactobacillus leich

mannii ATCC 4797, and Lactobacillus viridescens

ATCC 12706 used in the microbiological assay of vi

tamins.

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