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培養基配方

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Lactic Bacteria Broth

[所屬分類:培養基配方] [發布時間:2021-11-25] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Sodium acetate..................................................12.0g

Glucose .............................................................10.0g

Pancreatic digest of casein................................10.0g

Yeast autolysate ..................................................5.0g

Solution A....................................................... 5.0mL

Solution B ....................................................... 5.0mL

pH 5.1–5.3 at 25°C

Solution A:

Composition per 100.0mL:

K2HPO4.............................................................10.0g

KH2PO4.............................................................10.0g

Preparation of Solution A: Add components to

distilled/deionized water and bring volume to

100.0mL. Mix thoroughly.

Solution B:

Composition per 100.0mL:

MgSO4·7H2O......................................................4.0g

FeSO4..................................................................0.2g

MnSO4·5H2O......................................................0.2g

NaCl....................................................................0.2g

Preparation of Solution B: Add components to

distilled/deionized water and bring volume to

100.0mL. Mix thoroughly.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Lacto

bacillus buchneri, Lactobacillus delbrueckii, and Pe

diococcus damnosus.