- 定做培養基/定制培養基
- 顆粒培養基
- 標準菌株生化鑒定試劑盒
- 預灌裝即用型成品培養基
- 2025年版中國藥典
- 促銷/特價商品
- 院感/疾控/體外診斷/采樣管
- 樣品采集與處理(均質)產品
- 按標準檢索培養基
- 模擬灌裝用培養基
- 干燥粉末培養基
- 培養基添加劑/補充劑
- 生化反應鑒定管
- 染色液等配套產品
- 對照培養基/標準品
- 實驗耗材與器具
- 生化試劑/化學試劑
- 菌種鑒定服務
培養基配方
您現在的位置： 網站首頁 >> 培養基配方
Lactic Agar for Yogurt Bacteria, Modified
[所屬分類:培養基配方] [發布時間:2021-11-25] [發布人:網站管理員2] [閱讀次數:] [返回]
Lactic Agar for Yogurt
Bacteria, Modified
山東拓普生物工程有限公司 培養基配方 //ljxinglong.com
Composition per liter:
Elliker agar......................................................... 1.0L
Milk solution................................................. 70.0mL
pH 6.8 ± 0.1 at 25°C
Elliker Agar:
Composition per liter:
Pancreatic digest of casein................................20.0g
Agar ..................................................................15.0g
Yeast extract......................................................10.0g
Gelatin.................................................................4.0g
Glucose ...............................................................3.0g
Ascorbic acid ......................................................2.5g
Lactose................................................................2.5g
NaCl....................................................................2.5g
Sodium acetate....................................................2.5g
Sucrose................................................................2.5g
Preparation of Elliker Agar: Add components
to distilled/deionized water and bring volume to
1.0L. Mix thoroughly. Gently heat and bring to boil
ing. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 45°–50°C.
Milk Solution:
Composition per 100.0mL:
Nonfat dry milk solids ......................................11.0g
Preparation of Milk Solution: Add nonfat dry
milk solids to distilled/deionized water and bring
volume to 100.0mL. Mix thoroughly. Autoclave for
12 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Preparation of Medium: Add 70.0mL of sterile
milk solution to 1.0L of cooled, sterile Elliker agar.
Mix thoroughly. Pour into sterile Petri dishes. Allow
plates to dry at 28°–30°C for 18–24 hr.
Use: For the cultivation of acidogenic microorgan
isms, especially Lactobacillus species and lactic
streptococci, from foods.
