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L15 Medium, Modified Leibovitz (BAM M73)

[所屬分類:培養基配方] [發布時間:2021-11-25] [發布人:網站管理員2] [閱讀次數:] [返回]
L15 Medium, Modified Leibovitz
(BAM M73)

山東拓普生物(wu)工程有(you)限(xian)公司 培養(yang)基配(pei)方 //ljxinglong.com

Composition per liter:

NaCl....................................................................8.0g
DL-Threonine.......................................................0.6g
Sodium pyruvate.................................................0.6g
DL-Alanine...........................................................0.5g
L-Arginine, free base ...........................................0.5g
KCl......................................................................0.4g
L-Asparagine·H2O ...............................................0.3g
L-Histidine, free base...........................................0.3g
L-Glutamine.........................................................0.3g
L-Isoleucine .........................................................0.3g
L-Phenylalanine...................................................0.3g
L-Tyrosine............................................................0.3g
DL-Methionine.....................................................0.2g
DL-Valine .............................................................0.2g
Glycine................................................................0.2g
L-Serine ...............................................................0.2g
Na2HPO4, anhydrous ..........................................0.2g
CaCl2, anhydrous ................................................0.1g
L-Cysteine, free base ...........................................0.1g
L-Leucine·HCl.....................................................0.1g
MgCl2, anhydrous ...........................................0.094g
D-Galactose .......................................................0.09g
KH2PO4.............................................................0.06g
L-Tryptophan.....................................................0.02g
Phenol Red........................................................0.01g
i-Inositol...........................................................2.0mg
Choline chloride...............................................1.0mg
D-Calcium pantothenate ...................................1.0mg
Folic acid..........................................................1.0mg
Nicotinamide....................................................1.0mg
Pyridoxine·HCl ................................................1.0mg
Thiamine monophosphate·2H2O......................1.0mg
Riboflavin-5-phosphate ...................................0.1mg
pH 7.5 ± 0.2 at 25°C
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Filter sterilize. Store at 5°C.
Use: For the cultivation of oysters used for the
growth of enteroviruses.
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